Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell lines.
STAR Protoc
; 5(2): 103106, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38824641
ABSTRACT
Microfluidic single-cell cultivation (MSCC) is a powerful tool for investigating the cellular behavior of various cell types at the single-cell level. Here, we present a protocol specifically developed for the reliable and reproducible MSCC of industrially relevant Chinese hamster ovary (CHO) suspension cell lines. We summarize critical experimental steps from the initial seed train up to the final MSCC experiment, with a special focus on pre-culture management and medium preparation, device inoculation, and the establishment of a constant medium perfusion.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cricetulus
/
Técnicas de Cultivo de Célula
/
Análisis de la Célula Individual
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos