Female sample screening using colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) targeting non-coding RNA XIST.
Forensic Sci Int
; 361: 112081, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38850616
ABSTRACT
Forensic sample screening is important for establishing an effective DNA typing workflow. The detection of sex-specific markers in forensic samples highlights the necessity for further analysis. Y-chromosome DNA can confirm male contributions, but female contributions are difficult to confirm using DNA-based methods. To address this, we developed a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the long non-coding RNA X-inactive specific transcript (XIST) to screen female samples. Operating at 65⯰C for 30â¯min, the assay yielded results discernible from the color change of the pH indicator dye. The assay showed a detection limit of approximately 0.5⯵L of blood. The assay also detected XIST RNA in mixed body fluids and mock samples, indicating its potential applicability to casework samples. Taken together, our assay provides a rapid and simple strategy for screening female samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colorimetría
/
Técnicas de Amplificación de Ácido Nucleico
/
ARN Largo no Codificante
Límite:
Female
/
Humans
Idioma:
En
Revista:
Forensic Sci Int
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Irlanda