Open cranium model for the study of cerebrovascular dynamics in intracranial hypertension.
J Neurosci Methods
; 409: 110196, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-38880344
ABSTRACT
BACKGROUND:
Significant research has been devoted to developing noninvasive approaches to neuromonitoring. Clinical validation of such approaches is often limited, with minimal data available in the clinically relevant elevated ICP range. NEWMETHOD:
To allow ultrasound-guided placement of an intraventricular catheter and to perform simultaneous long-duration ICP and ultrasound recordings of cerebral blood flow, we developed a large unilateral craniectomy in a swine model. We also used a microprocessor-controlled actuator for intraventricular saline infusion to reliably and reversibly manipulate ICP according to pre-determined profiles.RESULTS:
The model was reproducible, resulting in over 80â¯hours of high-fidelity, multi-parameter physiological waveform recordings in twelve animals, with ICP ranging from 2 to 78â¯mmHg. ICP elevations were reversible and reproducible according to two predetermined profiles a stepwise elevation up to an ICP of 30-35â¯mmHg and return to normotension, and a clinically significant plateau wave. Finally, ICP was elevated to extreme levels of greater than 60â¯mmHg, simulating extreme clinical emergency. COMPARISON WITH EXISTINGMETHODS:
Existing methods for ICP monitoring in large animals typically relied on burr-hole approaches for catheter placement. Accurate catheter placement can be difficult in pigs, given the thickness of their skull. Additionally, ultrasound is significantly attenuated by the skull. The open cranium model overcomes these limitations.CONCLUSIONS:
The hemicraniectomy model allowed for verified placement of the intraventricular catheter, and reversible and reliable ICP manipulation over a wide range. The large dural window additionally allowed for long-duration recording of cerebral blood flow velocity from the middle cerebral artery.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Presión Intracraneal
/
Circulación Cerebrovascular
/
Hipertensión Intracraneal
/
Modelos Animales de Enfermedad
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Países Bajos