Caste-biased patterns of brain investment in the subterranean termite Reticulitermes flavipes.

ABSTRACT

Investment into neural tissue is expected to reflect the specific sensory and behavioral capabilities of a particular organism. Termites are eusocial insects that exhibit a caste system in which individuals can develop into one of several morphologically and behaviorally distinct castes. However, it is unclear to what extent these differences between castes are reflected in the anatomy of the brain. To address this question, we used deformation-based morphometry to conduct pairwise comparisons between the brains of different castes in the eastern subterranean termite, Reticulitermes flavipes. Workers exhibited enlargement in the antennal lobes and mushroom bodies, while reproductives showed increased investment into the optic lobes and central body. In addition, caste-specific enlargement was observed in regions that could not be mapped to distinct neuropils, most notably in soldiers. These findings demonstrate a significant influence of caste development on brain anatomy in termites alongside convergence with eusocial hymenopteran systems.