Analysis of the Nasal Microbiota in Healthy and Diseased Pigs.
Methods Mol Biol
; 2815: 93-113, 2024.
Article
en En
| MEDLINE
| ID: mdl-38884913
ABSTRACT
Massive sequencing of a fragment of 16S rRNA gene allows the characterization of bacterial communities in different body sites the microbiota. Nasal microbiota can be analyzed by DNA extraction from nasal swabs, amplification of the specific fragment of interest, and posterior sequencing. The raw sequences obtained need to go through a computational process to check their quality and then assign the taxonomy. Here, we will describe the complete process from sampling to get the microbial diversity of nasal microbiota in health and disease.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Ribosómico 16S
/
Microbiota
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos