Integrated transcriptome and metabolomics analyses revealed key functional genes in Canna indica under Cr stress.

ABSTRACT

Chromium (Cr) can interfere with plant gene expression, change the content of metabolites and affect plant growth. However, the molecular response mechanism of wetland plants at different time sequences under Cr stress has yet to be fully understood. In this study, Canna indica was exposed to 100 mg/kg Cr-contaminated soil for 0, 7, 14, and 21 days and analyzed using untargeted metabolomics (LC-MS) and transcriptomics. The results showed that Cr stress increased the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD), the contents of glutathione (GSH), malondialdehyde (MDA), and oxygen free radical (ROS), and inhibited the biosynthesis of photosynthetic pigments, thus leading to changes in plant growth and biomass. Metabonomics analysis showed that Cr stress mainly affected 12 metabolic pathways, involving 38 differentially expressed metabolites, including amino acids, phenylpropane, and flavonoids. By transcriptome analysis, a total of 16,247 differentially expressed genes (DEGs, 7710 up-regulated genes, and 8537 down-regulated genes) were identified, among which, at the early stage of stress (Cr contaminate seven days), C. indica responds to Cr toxicity mainly through galactose, starch and sucrose metabolism. With the extension of stress time, plant hormone signal transduction and MAPK signaling pathway in C. indica in the Cr14 (Cr contaminate 14 days) treatment group were significantly affected. Finally, in the late stage of stress (Cr21), C. indica co-defuses Cr toxicity by activating its Glutathione metabolism and Phenylpropanoid biosynthesis. In conclusion, this study revealed the molecular response mechanism of C. indica to Cr stress at different times through multi-omics methods.