Dual-mode biosensor using Tb-Cu MOF@Au nanoenzyme to effectively quench the photocurrent of Bi2O3/Bi2S3/AgBiS2 heterojunction and emit fluorescence for neuron-specific enolases detection.
Talanta
; 277: 126346, 2024 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-38897010
ABSTRACT
A novel dual-mode biosensor was constructed for the ultrasensitive detection of neuron-specific enolase (NSE), utilizing Tb-Cu MOF@Au nanozyme as the signal label to effectively quench the photoelectrochemical (PEC) signals of Bi2O3/Bi2S3/AgBiS2 composites and initiate fluorescent (FL) signals. First, Bi2O3/Bi2S3/AgBiS2 heterojunction with excellent photoelectric activity was selected as the substrate material to provide a stable photocurrent. The well-matched energy levels significantly enhanced the separation and transfer of photogenerated carriers. Second, a strategy of consuming ascorbic acid (AA) by Tb-Cu MOF@Au nanozyme was introduced to improve the sensitivity of the PEC/FL biosensor. Tb-Cu MOF@Au not only could catalyze the oxidation of AA, but the steric effect further reduced the contact of AA with the substrate. More importantly, in the presence of H2O2, a significant fluorescence was produced from Tb3+ sensitized by the oxidation products of AA. Based on the above strategies, a highly stable and sensitive dual-mode biosensor was proposed for accurate NSE determination. Third, the developed dual-mode biosensor demonstrated excellent performance in detecting NSE. In this study, the PEC method demonstrated a wide detection range from 0.00005 to 200 ng/mL with a low detection limit of 20 fg/mL. The FL method exhibited a linear range from 0.001 to 200 ng/mL with a detection limit of 0.65 pg/mL. The designed biosensor showed potential practical implications in the accurate detection of disease markers.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfopiruvato Hidratasa
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Terbio
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Bismuto
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Técnicas Biosensibles
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Cobre
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Oro
Límite:
Humans
Idioma:
En
Revista:
Talanta
Año:
2024
Tipo del documento:
Article
Pais de publicación:
HOLANDA
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HOLLAND
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NETHERLANDS
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NL
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PAISES BAJOS
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THE NETHERLANDS