Activated AMP-protein kinase (pAMPK) is overexpressed in human somatotroph pituitary adenomas.
Mol Cell Endocrinol
; 592: 112318, 2024 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-38908427
ABSTRACT
INTRODUCTION:
AMPK (AMP-activated protein kinase) is an enzyme that acts as a metabolic sensor and regulates multiple pathways via phosphorylating proteins in metabolic and proliferative pathways. The aim of this work was to study the activated cellular AMPK (phosphorylated-AMPK at Thr172, pAMPK) levels in pituitary tumor samples from patients with sporadic and familial acromegaly, as well as in samples from normal human pituitary gland.METHODS:
We studied pituitary adenoma tissue from patients with sporadic somatotroph adenomas, familial acromegaly with heterozygote germline variants in the aryl hydrocarbon receptor interacting protein (AIP) gene (p.Q164*, p.R304* and p.F269_H275dup) and autopsy from normal pituitary glands without structural alterations.RESULTS:
Cellular levels of pAMPK were significantly higher in patients with sporadic acromegaly compared to normal pituitary glands (p < 0.0001). Tissues samples from patients with germline AIP mutations also showed higher cellular levels of pAMPK compared to normal pituitary glands. We did not observe a significant difference in cellular levels of pAMPK according to the cytokeratin (CAM5.2) pattern (sparsely or densely granulated) for tumor samples of sporadic acromegaly.CONCLUSION:
Our data show, for the first time in human cells, an increase of cellular levels of pAMPK in sporadic somatotropinomas, regardless of cytokeratin pattern, as well as in GH-secreting adenomas from patients with germline AIP mutations.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adenoma
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Adenoma Hipofisario Secretor de Hormona del Crecimiento
/
Proteínas Quinasas Activadas por AMP
Límite:
Adult
/
Aged
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Mol Cell Endocrinol
/
Mol. cell. endocrinol
/
Molecular and cellular endocrinology
Año:
2024
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Irlanda