PfAgo-based dual signal amplification biosensor for rapid and highly sensitive detection of alkaline phosphatase activity.
Mikrochim Acta
; 191(7): 439, 2024 07 02.
Article
en En
| MEDLINE
| ID: mdl-38954110
ABSTRACT
A Pyrococcus furiosus Argonaute (PfAgo)-based biosensor is presented for alkaline phosphatase (ALP) activity detection in which the ALP-catalyzed hydrolysis of 3'-phosphate-modified functional DNA activates the strand displacement amplification, and the amplicon mediates the fluorescent reporter cleavage as a guide sequence of PfAgo. Under the dual amplification mode of PfAgo-catalyzed multiple-turnover cleavage activity and pre-amplification technology, the developed method was successfully applied to ALP activity determination with a detection limit (LOD) of 0.0013 U L-1 (3σ) and a detection range of 0.0025 to 1 U L-1 within 90 min. The PfAgo-based method exhibits satisfactory analytic performance in the presence of potential interferents and in complex human serum samples. The proposed method shows several advantages, such as rapid analysis, high sensitivity, low-cost, and easy operation, and has great potential in disease evolution fundamental studies and clinical diagnosis applications.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
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Pyrococcus furiosus
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Fosfatasa Alcalina
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Límite de Detección
Límite:
Humans
Idioma:
En
Revista:
Mikrochim Acta
Año:
2024
Tipo del documento:
Article
País de afiliación:
China