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The antagonistic role of an E3 ligase pair in regulating plant NLR-mediated autoimmunity and fungal pathogen resistance.
Liu, Jun; Yang, Yong; Ortiz-Morea, Fausto Andres; Zhou, Yulu; Liu, Derui; Huang, Yanyan; Zheng, Jiarui; Chen, Yan; Kong, Liang; Liu, Zunyong; Ge, Dongdong; Yong, Mingli; Lin, Wenwei; Russinova, Eugenia; Shan, Libo; He, Ping.
Afiliación
  • Liu J; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA; State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
  • Yang Y; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Ortiz-Morea FA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA; Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Amazonian Research Center Cimaz-Macagual, University of the Amazon, Florencia 18000
  • Zhou Y; Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Liu D; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA; Department of Plant Biotechnology & Bioinformatics, Ghent University, and Center for Plant Systems Biology, VIB, Ghent 9052, Belgium.
  • Huang Y; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Zheng J; State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
  • Chen Y; State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
  • Kong L; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA; Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Liu Z; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA; Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Ge D; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Yong M; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Lin W; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • Russinova E; Department of Plant Biotechnology & Bioinformatics, Ghent University, and Center for Plant Systems Biology, VIB, Ghent 9052, Belgium.
  • Shan L; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA; Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
  • He P; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA; Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: pinghemi@umich.edu.
Cell Host Microbe ; 32(7): 1114-1128.e10, 2024 Jul 10.
Article en En | MEDLINE | ID: mdl-38955187
ABSTRACT
Plant immune homeostasis is achieved through a balanced immune activation and suppression, enabling effective defense while averting autoimmunity. In Arabidopsis, disrupting a mitogen-activated protein (MAP) kinase cascade triggers nucleotide-binding leucine-rich-repeat (NLR) SUPPRESSOR OF mkk1/2 2 (SUMM2)-mediated autoimmunity. Through an RNAi screen, we identify PUB5, a putative plant U-box E3 ligase, as a critical regulator of SUMM2-mediated autoimmunity. In contrast to typical E3 ligases, PUB5 stabilizes CRCK3, a calmodulin-binding receptor-like cytoplasmic kinase involved in SUMM2 activation. A closely related E3 ligase, PUB44, functions oppositely with PUB5 to degrade CRCK3 through monoubiquitylation and internalization. Furthermore, CRCK3, highly expressed in roots and conserved across plant species, confers resistance to Fusarium oxysporum, a devastating soil-borne fungal pathogen, in both Arabidopsis and cotton. These findings demonstrate the antagonistic role of an E3 ligase pair in fine-tuning kinase proteostasis for the regulation of NLR-mediated autoimmunity and highlight the function of autoimmune activators in governing plant root immunity against fungal pathogens.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Autoinmunidad / Arabidopsis / Proteínas de Arabidopsis / Ubiquitina-Proteína Ligasas / Inmunidad de la Planta / Resistencia a la Enfermedad / Fusarium Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Autoinmunidad / Arabidopsis / Proteínas de Arabidopsis / Ubiquitina-Proteína Ligasas / Inmunidad de la Planta / Resistencia a la Enfermedad / Fusarium Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos