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Stereospecific reduction of 2'S-configured strigolactones by cowpea OPR3 enzymes.
Suzawa, Shota; Yamauchi, Misa; Homma, Masato; Yamauchi, Yasuo; Mizutani, Masaharu; Wakabayashi, Takatoshi; Sugimoto, Yukihiro.
Afiliación
  • Suzawa S; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Japan.
  • Yamauchi M; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Japan.
  • Homma M; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Japan.
  • Yamauchi Y; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Japan.
  • Mizutani M; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Japan.
  • Wakabayashi T; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Japan.
  • Sugimoto Y; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Biosci Biotechnol Biochem ; 88(10): 1172-1179, 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-38970383
ABSTRACT
Strigolactones (SLs), plant-derived apocarotenoids, serve dual roles as phytohormones and rhizosphere signaling molecules. While exogenous administration of SLs to plants aids in studying their functions, the metabolic destiny of these administered SLs remains poorly elucidated. Our previous research demonstrated that among synthetic SL GR24 stereoisomers administered to cowpea (Vigna unguiculata), 2'-epi-GR24 undergoes selective reduction at the C-3',4' double bond in its D-ring. In this investigation, we isolated proteins from cowpea roots based on SL reducing activity and identified 12-oxophytodienoate reductase 3 homologs (VuOPR3s) as contributors to this reduction. Enzymatic assays conducted with recombinant proteins revealed that VuOPR3s exhibited a preference for reducing activity toward 2'S-configured SLs, including 2'-epi-GR24. This specificity for 2'S-configured SLs was congruent with that observed for orobanchol produced by cowpea and its stereoisomers. These findings suggest that exogenously administered SLs undergo enzymatic stereoselective reduction, underscoring the importance of considering stereospecificity when interpreting data obtained from SL usage.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxidación-Reducción / Vigna / Lactonas Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxidación-Reducción / Vigna / Lactonas Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido