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A comparison of antibiotic resistance genes and mobile genetic elements in wild and captive Himalayan vultures.
Zhai, Jundie; Wang, You; Tang, Boyu; Zheng, Sisi; He, Shunfu; Zhao, Wenxin; Lin, Jun; Li, Feng; Bao, Yuzi; Lancuo, Zhuoma; Liu, Chuanfa; Wang, Wen.
Afiliación
  • Zhai J; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
  • Wang Y; College of Eco-Environmental Engineering, Qinghai University, Xining, China.
  • Tang B; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
  • Zheng S; College of Eco-Environmental Engineering, Qinghai University, Xining, China.
  • He S; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
  • Zhao W; College of Eco-Environmental Engineering, Qinghai University, Xining, China.
  • Lin J; Animal Disease Prevention and Control Center of Qinghai Province, Xining, China.
  • Li F; Xining Wildlife Park of Qinghai Province, Xining, China.
  • Bao Y; Xining Wildlife Park of Qinghai Province, Xining, China.
  • Lancuo Z; College of Eco-Environmental Engineering, Qinghai University, Xining, China.
  • Liu C; College of Eco-Environmental Engineering, Qinghai University, Xining, China.
  • Wang W; College of Eco-Environmental Engineering, Qinghai University, Xining, China.
PeerJ ; 12: e17710, 2024.
Article en En | MEDLINE | ID: mdl-39006014
ABSTRACT
As the most widely distributed scavenger birds on the Qinghai-Tibetan Plateau, Himalayan vultures (Gyps himalayensis) feed on the carcasses of various wild and domestic animals, facing the dual selection pressure of pathogens and antibiotics and are suitable biological sentinel species for monitoring antibiotic resistance genes (ARGs). This study used metagenomic sequencing to comparatively investigate the ARGs and mobile genetic elements (MGEs) of wild and captive Himalayan vultures. Overall, the resistome of Himalayan vultures contained 414 ARG subtypes resistant to 20 ARG types, with abundances ranging from 0.01 to 1,493.60 ppm. The most abundant resistance type was beta-lactam (175 subtypes), followed by multidrug resistance genes with 68 subtypes. Decreases in the abundance of macrolide-lincosamide-streptogramin (MLS) resistance genes were observed in the wild group compared with the zoo group. A total of 75 genera (five phyla) of bacteria were predicted to be the hosts of ARGs in Himalayan vultures, and the clinical (102 ARGs) and high-risk ARGs (35 Rank I and 56 Rank II ARGs) were also analyzed. Among these ARGs, twenty-two clinical ARGs, nine Rank I ARG subtypes, sixteen Rank II ARG subtypes were found to differ significantly between the two groups. Five types of MGEs (128 subtypes) were found in Himalayan vultures. Plasmids (62 subtypes) and transposases (44 subtypes) were found to be the main MGE types. Efflux pump and antibiotic deactivation were the main resistance mechanisms of ARGs in Himalayan vultures. Decreases in the abundance of cellular protection were identified in wild Himalayan vultures compared with the captive Himalayan vultures. Procrustes analysis and the co-occurrence networks analysis revealed different patterns of correlations among gut microbes, ARGs, and MGEs in wild and captive Himalayan vultures. This study is the first step in describing the characterization of the ARGs in the gut of Himalayan vultures and highlights the need to pay more attention to scavenging birds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Secuencias Repetitivas Esparcidas / Animales Salvajes Límite: Animals País/Región como asunto: Asia Idioma: En Revista: PeerJ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Secuencias Repetitivas Esparcidas / Animales Salvajes Límite: Animals País/Región como asunto: Asia Idioma: En Revista: PeerJ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos