Protocol to study early lung developmental branching in mouse embryos using explant culture.
STAR Protoc
; 5(3): 103198, 2024 Jul 17.
Article
en En
| MEDLINE
| ID: mdl-39024094
ABSTRACT
Mouse lung branching morphogenesis creates epithelial tree structures required for respiration. Here, we present a protocol for studying mouse lung developmental branching using lung explant cultures. We describe steps for isolating lungs with a video at embryonic day 12.5 (E12.5) and culturing as an explant for 2 days. We also detail procedures for microscopic imaging on days 0-2 and analysis of peripheral lung buds. This technique has the potential to investigate lung development in various conditions. For complete details on the use and execution of this protocol, please refer to Talvi et al.1.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
Pais de publicación:
EEUU
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ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA