Microwave seed priming and ascorbic acid assisted phytoextraction of heavy metals from surgical industry effluents through spinach.
Ecotoxicol Environ Saf
; 282: 116731, 2024 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-39029219
ABSTRACT
The prevalence of inorganic pollutants in the environment, including heavy metals (HMs), necessitates a sustainable and cost-effective solution to mitigate their impacts on the environment and living organisms. The present research aimed to assess the phytoextraction capability of spinach (Spinach oleracea L.), under the combined effects of ascorbic acid (AA) and microwave (MW) irradiation amendments, cultivated using surgical processing wastewater. In a preliminary study, spinach seeds were exposed to MW radiations at 2.45â¯GHz for different durations (15, 30, 45, 60, and 90â¯seconds). Maximum germination was observed after the 30â¯seconds of radiation exposure. Healthy spinach seeds treated with MW radiations for 30â¯s were cultivated in the sand for two weeks, after which juvenile plants were transferred to a hydroponic system. Surgical industry wastewater in different concentrations (25â¯%, 50â¯%, 75â¯%, 100â¯%) and AA (10â¯mM) were provided to both MW-treated and untreated plants. The results revealed that MW-treatment significantly enhanced the plant growth, biomass, antioxidant enzyme activities and photosynthetic pigments, while untreated plants exhibited increased reactive oxygen species (ROS) and electrolyte leakage (EL) compared with their controls. The addition of AA to both MW-treated and untreated plants improved their antioxidative defense capacity under HMs-induced stress. MW-treated spinach plants, under AA application, demonstrated relatively higher concentrations and accumulation of HMs including lead (Pb), cadmium (Cd) and nickel (Ni). Specifically, MW-treated plants with AA amendment showed a significant increase in Pb concentration by 188â¯% in leaves, Cd by 98â¯%, and Ni by 102â¯% in roots. Additionally, the accumulation of Ni increased by 174â¯% in leaves, Cd by 168â¯% in roots, and Pb by 185â¯% in the stem of spinach plant tissues compared to MW-untreated plants. These findings suggested that combining AA with MW irradiation of seeds could be a beneficial strategy for increasing the phytoextraction of HMs from wastewater and improving overall plant health undergoing HMs stress.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Ascórbico
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Semillas
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Biodegradación Ambiental
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Spinacia oleracea
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Metales Pesados
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Microondas
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Países Bajos