NDMNN: A novel deep residual network based MNN method to remove batch effects from scRNA-seq data.

ABSTRACT

The rapid development of single-cell RNA sequencing (scRNA-seq) technology has generated vast amounts of data. However, these data often exhibit batch effects due to various factors such as different time points, experimental personnel, and instruments used, which can obscure the biological differences in the data itself. Based on the characteristics of scRNA-seq data, we designed a dense deep residual network model, referred to as NDnetwork. Subsequently, we combined the NDnetwork model with the MNN method to correct batch effects in scRNA-seq data, and named it the NDMNN method. Comprehensive experimental results demonstrate that the NDMNN method outperforms existing commonly used methods for correcting batch effects in scRNA-seq data. As the scale of single-cell sequencing continues to expand, we believe that NDMNN will be a valuable tool for researchers in the biological community for correcting batch effects in their studies. The source code and experimental results of the NDMNN method can be found at https//github.com/mustang-hub/NDMNN.