Prostaglandin E2 regulates the plasminogen activator pathway in human endometrial endothelial cells: a new in vitro model to investigate heavy menstrual bleeding.
F S Sci
; 2024 Jul 20.
Article
en En
| MEDLINE
| ID: mdl-39038609
ABSTRACT
OBJECTIVE:
To study the role of PGE2 in regulating plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (tPA) in human primary endometrial endothelial cells (HEECs) from women with normal menstrual bleeding (NMB) and heavy menstrual bleeding (HMB).DESIGN:
In vitro study using endometrial endothelial cells.SETTING:
Research laboratory setting. PATIENTS Women with NMB and HMB provided endometrial biopsy samples.INTERVENTIONS:
Prostaglandin E2 and PGE2 receptor-selective agonists were administered to cultured HEECs. MAIN OUTCOMEMEASURES:
Levels of PAI-1 and tPA in NMB-HEECs and HMB-HEECs after treatment with PGE2 and receptor-selective agonists.RESULTS:
Prostaglandin E2 increased total PAI-1 levels in NMB-HEECs, but not in HMB-HEECs, which had higher baseline PAI-1 levels. PGE2 receptors (PTGER)1 and PTGER2 agonists increased PAI-1 in NMB-HEECs, whereas PTGER3 and PTGER4 did not. Prostaglandin E2 had no effect on tPA levels in either NMB-HEECs or HMB-HEECs.CONCLUSIONS:
Prostaglandin E2, through PTGER1 and PTGER2, regulates the plasminogen activator system in NMB-HEECs, suggesting a role in reducing fibrinolytic activity during normal menstrual cycles. The lack of PGE2 effect and elevated baseline PAI-1 in HMB-HEECs support using this in vitro model to further understand prostaglandin pathways in NMB and HMB.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
F S Sci
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos