Evaluation of the Nonlinear Response of KCl Solution to High-Amplitude Stimulation Using a Four-Point Cylindrical Gold Electrode.

ABSTRACT

A four-electrode system and high-voltage amplitude stimulation (with a voltage of more than 1 V) are always used for nonlinear spectroscopy of biological solutions like yeast suspension. Multiple studies indicate that yeast membrane activities are the primary source of the harmonics detected from yeast suspensions. However, other investigations have demonstrated that at least part of the harmonics are mainly due to the nonlinear impedance of the electrode-electrolyte interface. Here, we want to find a mathematical model to analyze the odd harmonics generated by the interface. The KCl solution is the basis of the essential medium used in yeast culture. No study models the nonlinear behavior of the KCl solution using four-electrode. We used a sinusoidal voltage with a low frequency and a high amplitude to test an ionic solution with 1, 30, and 130 mM of potassium chloride. The voltage had no direct current component. Here, we show that the relationship between the amplitude of the excitation voltage and the third harmonic of the current can be accurately represented using the Butler-Volmer equation. Additionally, we analyze the impact of potassium chloride (KCl) concentration on the behavior of the third harmonic. This paper presents a method and model that can be used for nonlinear spectroscopy studies of biological solutions using a four-electrode system with the aim of deriving information from cells without considering the interface effect.