Comparison among different preclinical models derived from the same patient with a non-functional pancreatic neuroendocrine tumor.
Hum Cell
; 37(5): 1522-1534, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-39078546
ABSTRACT
Pancreatic neuroendocrine tumors are the second most common tumors of the pancreas, and approximately half of patients are diagnosed with liver metastases. Currently, the improvement in the efficacy of relevant treatment methods is still limited. Therefore, there is an urgent need for in-depth research on the molecular biological mechanism of pancreatic neuroendocrine tumors. However, due to their relatively inert biology, preclinical models are extremely scarce. Here, the patient-derived organoid, and patient-derived xenograft were successfully constructed. These two models and the previously constructed cell line named SPNE1 all derived from the same patient with a grade 3 non-functional pancreatic neuroendocrine tumor, providing new tumor modeling platforms, and characterized using immunohistochemistry, whole-exome sequencing, and single-cell transcriptome sequencing. Combined with a tumor formation experiment in immunodeficient mice, we selected the model that most closely recapitulated the parental tumor. Overall, the patient-derived xenograft model most closely resembled human tumor tissue.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Pancreáticas
/
Tumores Neuroendocrinos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Hum Cell
/
Hum. cell
/
Human cell
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Japón