An arabinoxylan (AOP70-1) isolated from Alpinia oxyphylla alleviates neuroinflammation and neurotoxicity by TLR4/MyD88/NF-κB pathway.
Int J Biol Macromol
; 277(Pt 3): 134339, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39089558
ABSTRACT
Alpinia oxyphylla is famous for its neuroprotective and memory-improving effects. A crude polysaccharide AO70 from A. oxyphylla remarkably ameliorated neuroinflammation and cognitive dysfunction in Alzheimer's disease mice. This study aimed to explore the bioactive component of AO70 and its mechanism of action. A homogeneous polysaccharide (AOP70-1) rich in arabinose and xylose was purified from AO70, which was consisted of α-L-Araf-(1â, â5)-α-L-Araf-(1â, ß-D-Xylp-(1â,â2,4)-ß-D-Xylp-(1â, â2,3,4)-ß-D-Xylp-(1â, α-L-Rhap-(1â, α-D-Manp-(1â, â4)-α-D-Glcp-(1â, â4)-α-D-GlcpA-(1â, ß-D-Galp-(1â, â2)-α-D-Galp-(1â, â6)-α-D-Galp-(1 â and â3,6)-α-D-Manp-(1 â. AOP70-1 (2.5, 5, 10 µM) significantly suppressed NO, IL-1ß, and TNF-α production in a concentration-dependent manner and inhibited the migration of BV2 microglia. AOP70-1 inhibited LPS-mediated activation of Toll-like receptor 4 (TLR4), myeloid differentiation primary response protein (MyD88), and nuclear factor kappa B (NF-κB). Moreover, AOP70-1 exerted neuroprotection on SH-SY5Y cells and primary neurons by reducing neuronal apoptosis (72 %, 44 %), alleviating ROS accumulation (63 %, 55 %), and improving mitochondrial membrane potential (63 %, 77 %). Overall, AOP70-1 is one of the major bioactive components in AO70 from A. oxyphylla, which has great potential in the prevention and treatment of neuroinflammation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Xilanos
/
Transducción de Señal
/
Alpinia
/
Enfermedades Neuroinflamatorias
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos