Microbacterium humicola sp. nov., Microbacterium terrisoli sp. nov., Paenibacillus pedocola sp. nov., Paenibacillus silviterrae sp. nov., Flavobacterium terrisoli sp. nov., and Aquabacterium humicola sp. nov., isolated from soil.
Int J Syst Evol Microbiol
; 74(8)2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-39120518
ABSTRACT
Four Gram-stain-positive and two Gram-stain-negative bacterial strains, designated as W4T, FW7T, TW48T, UW52T, PT-3T, and RJY3T, were isolated from soil samples collected from the Republic of Korea. The 16S rRNA gene sequence analysis showed that strains W4T and FW7T belonged to the genus Microbacterium, strains TW48T and UW52T were affiliated to the genus Paenibacillus, strain PT-3T was related to the genus Flavobacterium, and strain RJY3T was associated with the genus Aquabacterium. The closest phylogenetic taxa to W4T, FW7T, TW48T, UW52T, PT-3T, and RJY3T were Microbacterium bovistercoris NEAU-LLET (97.7â%), Microbacterium protaetiae DFW100M-13T (97.9â%), Paenibacillus auburnensis JJ-7T (99.6â%), Paenibacillus allorhizosphaerae JJ-447T (95.7â%), Flavobacterium buctense T7T (97.1â%), and Aquabacterium terrae S2T (99.5â%), respectively. Average nucleotide identity and digital DNA-DNA hybridization values between the novel strains and related reference type strains were <95.0â% and <70.0â%, respectively. The major cellular fatty acid in strains W4T, FW7T TW48T, and UW52T was antiso-C15ââ0. Similarly, strain PT-3T revealed iso-C15ââ0, iso-C15ââ1 G, iso-C17ââ0 3-OH, and iso-C15ââ0 3-OH as its principal fatty acids. On the other hand, RJY3T exhibited summed feature 3 (C16ââ1 ω7c and/or C16ââ1 ω6c), C16ââ0, summed feature 8 (C18ââ1 ω7c and/or C18ââ1 ω6c), and C12ââ0 as its predominant fatty acids. Overall, the polyphasic taxonomic data indicated that strains W4T, FW7T, TW48T, UW52T, PT-3T, and RJY3T represent novel species within the genera Microbacterium, Paenibacillus, Flavobacterium, and Aquabacterium. Accordingly, we propose the names Microbacterium humicola sp. nov., with the type strain W4T (=KCTC 49888T=NBRC 116001T), Microbacterium terrisoli sp. nov., with the type strain FW7T (=KCTC 49859T=NBRC 116000T), Paenibacillus pedocola sp. nov., with the type strain TW48T (=KCTC 43470T=NBRC 116017T), Paenibacillus silviterrae sp. nov., with the type strain UW52T (=KCTC 43477T=NBRC 116018T), Flavobacterium terrisoli sp. nov., with the type strain PT-3T (=KCTC 92106T=NBRC 116012T), and Aquabacterium humicola sp. nov., with the type strain RJY3T (=KCTC 92105T=NBRC 115831T).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Filogenia
/
Microbiología del Suelo
/
Composición de Base
/
ADN Bacteriano
/
ARN Ribosómico 16S
/
Flavobacterium
/
Técnicas de Tipificación Bacteriana
/
Análisis de Secuencia de ADN
/
Ácidos Grasos
/
Paenibacillus
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2024
Tipo del documento:
Article