Inferring clonal somatic mutations directed by X chromosome inactivation status in single cells.
Genome Biol
; 25(1): 214, 2024 Aug 09.
Article
en En
| MEDLINE
| ID: mdl-39123248
ABSTRACT
Analysis of clonal dynamics in human tissues is enabled by somatic genetic variation. Here, we show that analysis of mitochondrial mutations in single cells is dramatically improved in females when using X chromosome inactivation to select informative clonal mutations. Applying this strategy to human peripheral mononuclear blood cells reveals clonal structures within T cells that otherwise are blurred by non-informative mutations, including the separation of gamma-delta T cells, suggesting this approach can be used to decipher clonal dynamics of cells in human tissues.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inactivación del Cromosoma X
/
Análisis de la Célula Individual
/
Mutación
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Reino Unido