Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus.
Methods Mol Biol
; 2838: 211-219, 2024.
Article
en En
| MEDLINE
| ID: mdl-39126635
ABSTRACT
Next-generation sequencing (NGS) technologies are continuously being developed and are becoming a more cost-effective tool for the characterization of viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary mechanisms as genetic reassortment. Here, we present a detailed method for obtaining full genome sequence data for EHDV using Illumina technology. The protocol includes details from RNA extraction and purification, the synthesis of cDNA, sequencing library preparation, to genome assembly.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genoma Viral
/
Virus de la Enfermedad Hemorrágica Epizoótica
/
Secuenciación de Nucleótidos de Alto Rendimiento
/
Secuenciación Completa del Genoma
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
Reino Unido