A comparison of likelihood ratios calculated from surface DNA mixtures using MPS and CE Technologies.
Forensic Sci Int Genet
; 73: 103111, 2024 Nov.
Article
en En
| MEDLINE
| ID: mdl-39128429
ABSTRACT
This study evaluates the performance of analysing surface DNA samples using massively parallel sequencing (MPS) compared to traditional capillary electrophoresis (CE). A total of 30 samples were collected from various surfaces in an office environment and were analysed with CE and MPS. These were compared against 60 reference samples (office inhabitants). To identify contributors, likelihood ratios (LRs) were calculated for MPS and CE data using the probabilistic genotyping software MPSproto and EuroForMix respectively. Although a higher number of sequences/peaks were observed per DNA profile in MPS compared to CE, LR values were found to be lower for MPS data formats. This might be the result of the increased complexity of MPS data, along with a possible elevation of unknown alleles and/or artefacts. The study highlights avenues for improving MPS data quality and analysis to facilitate more robust interpretation of challenging casework-like samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Dermatoglifia del ADN
/
Electroforesis Capilar
/
Secuenciación de Nucleótidos de Alto Rendimiento
Límite:
Humans
Idioma:
En
Revista:
Forensic Sci Int Genet
Asunto de la revista:
GENETICA
/
JURISPRUDENCIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Noruega
Pais de publicación:
Países Bajos