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Interaction of Acinetobacter sp. RIT 592 induces the production of broad-spectrum antibiotics in Exiguobacterium sp. RIT 594.
Parthasarathy, Anutthaman; Miranda, Renata Rezende; Bedore, T J; Watts, Lizabeth M; Mantravadi, Pavan K; Wong, Narayan H; Chu, Jonathan; Adjei, Joseph A; Rana, Amisha P; Savka, Michael A; Bulman, Zackery P; Borrego, Eli J; Hudson, André O.
Afiliación
  • Parthasarathy A; School of Chemistry and Biosciences, University of Bradford, Bradford, United Kingdom.
  • Miranda RR; School of Chemistry and Materials Science, Rochester Institute of Technology, Rochester, NY, United States.
  • Bedore TJ; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
  • Watts LM; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
  • Mantravadi PK; Agilent Technologies, Santa Clara, CA, United States.
  • Wong NH; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
  • Chu J; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
  • Adjei JA; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
  • Rana AP; Department of Pharmacy Practice, University of Illinois at Chicago, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, United States.
  • Savka MA; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
  • Bulman ZP; Department of Pharmacy Practice, University of Illinois at Chicago, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, United States.
  • Borrego EJ; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
  • Hudson AO; Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.
Front Pharmacol ; 15: 1456027, 2024.
Article en En | MEDLINE | ID: mdl-39148551
ABSTRACT
Antimicrobial resistance (AMR) is one of the most alarming global public health challenges of the 21st century. Over 3 million antimicrobial-resistant infections occur in the United States annually, with nearly 50,000 cases being fatal. Innovations in drug discovery methods and platforms are crucial to identify novel antibiotics to combat AMR. We present the isolation and characterization of potentially novel antibiotic lead compounds produced by the cross-feeding of two rhizosphere bacteria, Acinetobacter sp. RIT 592 and Exiguobacterium sp. RIT 594. We used solid-phase extraction (SPE) followed by liquid chromatography (LC) to enrich antibiotic extracts and subsequently mass spectrometry (MS) analysis of collected fractions for compound structure identification and characterization. The MS data were processed through the Global Natural Product Social Molecular Networking (GNPS) database. The supernatant from RIT 592 induced RIT 594 to produce a cocktail of antimicrobial compounds active against Gram-positive and negative bacteria. The GNPS analysis indicated compounds with known antimicrobial activity in the bioactive samples, including oligopeptides and their derivatives. This work emphasizes the utility of microbial community-based platforms to discover novel clinically relevant secondary metabolites. Future work includes further structural characterization and antibiotic activity evaluation of the individual compounds against pathogenic multidrug-resistant (MDR) bacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza