An acidic polysaccharide promoting GLP-1 secretion from Dendrobium huoshanense protocorm-like bodies: Structure validation and activity exploration.
Int J Biol Macromol
; 278(Pt 2): 134783, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39153673
ABSTRACT
Glucagon-like peptide-1 (GLP-1) as a multifunctional hormone is secreted mainly from enteroendocrine L-cells, and enhancing its endogenous secretion has potential benefits of regulating glucose homeostasis and controlling body weight gain. In the present study, a novel polysaccharide (h-DHP) with high ability to enhance plasma GLP-1 level in mice was isolated from Dendrobium huoshanense protocorm-like bodies under the guidance of activity evaluation. Structural identification showed that h-DHP was an acidic polysaccharide with the molecular weight of 1.38 × 105 Da, and was composed of galactose, glucose, arabinose and glucuronic acid at a molar ratio of 15.7 11.2 4.5 1.0 with a backbone consisting of â5)-α-L-Araf-(1â, â3)-α-D-Galp-(1â, â6)-α-D-Galp-(1â, â3,6)-α-D-Galp-(1â, â6)-ß-D-Glcp-(1â and â4,6)-ß-D-Glcp-(1â along with branches consisting of α-L-Araf-(1â, α-D-Galp-(1â, α-D-GlcAp-(1â, ß-D-Glcp-(1â and â4)-ß-D-Glcp-(1â. Animal experiments with different administration routes demonstrated that h-DHP-enhanced plasma GLP-1 level was attributed to h-DHP-promoted GLP-1 secretion in the enteroendocrine L-cells, which was supported by h-DHP-enhanced extracellular GLP-1 level in STC-1 cells. Inhibition of adenylate cyclase and phospholipase C indicated that cAMP and cAMP-triggered intracellular Ca2+ increase participated in h-DHP-promoted GLP-1 secretion. These results suggested that h-DHP has the potential of enhancing endogenous GLP-1 level through h-DHP-promoted and cAMP-mediated GLP-1 secretion from enteroendocrine cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Dendrobium
/
Péptido 1 Similar al Glucagón
Límite:
Animals
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Países Bajos