Multifunctional Tetrode-like Drug delivery, Optical stimulation, and Electrophysiology (Tetro-DOpE) probes.
Biosens Bioelectron
; 265: 116696, 2024 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-39208508
ABSTRACT
Having reliable tools for recording and manipulating circuit activity are essential to understand the complex patterns of neural dynamics that underlie brain function. We present Tetro-DOpE (Tetrode-like Drug delivery, Optical stimulation, and Electrophysiology) probes that can simultaneously record and manipulate neural activity in behaving rodents. We fabricated thin multifunctional fibers (<50 µm) using the scalable convergence thermal drawing process. Then, the thin fibers are bundled, similar to tetrode fabrication, to produce Tetro-DOpE probes. We demonstrated the multifunctionality (i.e., electrophysiology, optical stimulation, and drug delivery) of our probe in head-fixed behaving mice. Furthermore, we assembled a six-shank probe mounted on a microdrive which enabled stable recordings of over months when chronically implanted in freely behaving mice. These in vivo experiments demonstrate the potential of customizable, low cost, and accessible multifunctional Tetro-DOpE probes for investigation of neural circuitry in behaving animals.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sistemas de Liberación de Medicamentos
Límite:
Animals
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido