Transfer of liposome-encapsulated plasmid DNA to Bacillus subtilis protoplasts and calcium-treated Escherichia coli cells.
Folia Microbiol (Praha)
; 30(2): 97-100, 1985.
Article
en En
| MEDLINE
| ID: mdl-3922858
ABSTRACT
Protoplasts of Bacillus subtilis 168 trpC2 str and cells of Escherichia coli SK 1590 after treatment with calcium chloride were transformed to tetracycline resistance with the recombinant plasmid pUN82 entrapped in the reverse phase evaporation liposomes. Frequency of transfer was 4 X 10(-4)% in B. subtilis and 8 X 10(-6)% in E. coli.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Fosfatidilgliceroles
/
Protoplastos
/
Bacillus subtilis
/
Cloruro de Calcio
/
Factores R
/
Escherichia coli
/
Liposomas
Idioma:
En
Revista:
Folia Microbiol (Praha)
Año:
1985
Tipo del documento:
Article