Identifying Putative Biomarkers of Foodborne Pathogens Using a Metabolomic Approach.
Methods Mol Biol
; 2852: 255-272, 2025.
Article
en En
| MEDLINE
| ID: mdl-39235749
ABSTRACT
Metabolomics is the study of low molecular weight biochemical molecules (typically <1500 Da) in a defined biological organism or system. In case of food systems, the term "food metabolomics" is often used. Food metabolomics has been widely explored and applied in various fields including food analysis, food intake, food traceability, and food safety. Food safety applications focusing on the identification of pathogen-specific biomarkers have been promising. This chapter describes a nontargeted metabolite profiling workflow using gas chromatography coupled with mass spectrometry (GC-MS) for characterizing three globally important foodborne pathogens, Escherichia coli O157H7, Listeria monocytogenes, and Salmonella enterica, from selective enrichment liquid culture media. The workflow involves a detailed description of food spiking experiments followed by procedures for the extraction of polar metabolites from media, the analysis of the extracts using GC-MS, and finally chemometric data analysis using univariate and multivariate statistical tools to identify potential pathogen-specific biomarkers.
Palabras clave
Food safety; Foodborne pathogens; Gas chromatography-mass spectrometry (GC-MS); MetaboAnalyst; Metabolomic profiling; Partial least square-discriminant analysis (PLS-DA); Principal component analysis (PCA); Receiver operating characteristic area under the curve (ROC-AUC) analysis; SIMCA; Significant analysis of metabolomics (SAM) plots
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biomarcadores
/
Metabolómica
/
Microbiología de Alimentos
/
Listeria monocytogenes
/
Cromatografía de Gases y Espectrometría de Masas
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2025
Tipo del documento:
Article
País de afiliación:
Australia
Pais de publicación:
Estados Unidos