Protective Effects of Curcumin against Nephrotoxicity in Male Rats after Chronic Exposure to Chlorpyrifos.

ABSTRACT

BACKGROUND: Organophosphorus insecticides, widely used in farming and agriculture, have been associated with various health issues. Curcumin, a natural antioxidant, has shown potential in mitigating the adverse effects induced by these insecticides. AIM: This study aimed to evaluate the nephroprotective effects of Curcumin (CUR) against Chlorpyrifos (CPF)-induced renal damage. METHOD: Forty male Wistar albino rats were randomly assigned to five groups, each containing eight rats control (0.5 mL of olive oil, the solvent for chlorpyrifos), CPF (10 mg/kg of chlorpyrifos), CPF + CUR 25 mg/kg/day, CPF + CUR 50 mg/kg/day, and CPF + CUR 100 mg/k/day. All groups were treated for 90 days. Finally, kidney tissue was assessed for oxidative stress, inflammatory markers, and histopathological changes. RESULT: A considerable elevation in urea and Creatinine (Cr) concentrations was observed in the CPF group compared to the control rats (p < 0.01). CUR decreased creatinine and urea levels in the CPF-exposed group compared to the non-CUR-treated animals (p < 0.05). Additionally, the concentrations of NO, MDA, IL-6, IL-1ß, and TNFα were significantly increased in the kidneys of the CPF-induced rats compared to the controls (p < 0.001). However, CUR (100 mg/kg) administration significantly reduced the abovementioned parameters in rat kidneys (p < 0.01). CUR (100 mg/kg) also increased superoxide dismutase activity and glutathione concentration in the kidneys of CPF-exposed animals compared to non-CUR-treated rats (p < 0.05). Histopathological analysis revealed severe congestion in the kidney tissue after CPF exposure. However, coadministration of CUR restored the normal structure of the kidney in the experimental rats. CONCLUSION: Our findings suggest that curcumin, a potent antioxidant, can help alleviate chlorpyrifos-induced nephrotoxicity.