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Investigation of co-flow step emulsification (CFSE) microfluidic device and its applications in digital polymerase chain reaction (ddPCR).
Wei, Chunyang; Lv, Wei; Ding, Yanjing; Wang, Chen; Sun, Chengduo; Feng, Xinhang; Zhang, Tianqi; Li, Junwei; Li, Qinghua; Li, Shanshan.
Afiliación
  • Wei C; College of Medical Information and Artificial Intelligence, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.
  • Lv W; Department of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250399, China.
  • Ding Y; College of Medical Information and Artificial Intelligence, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.
  • Wang C; College of Medical Information and Artificial Intelligence, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.
  • Sun C; College of Medical Information and Artificial Intelligence, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.
  • Feng X; College of Medical Information and Artificial Intelligence, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.
  • Zhang T; College of Medical Information and Artificial Intelligence, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.
  • Li J; Institute of Biophysics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300401, China. Electronic address: junwei_li@hebut.edu.cn.
  • Li Q; College of Medical Information and Artificial Intelligence, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China; College of Radiology, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China. Electronic address:
  • Li S; Hebei Key Laboratory of Robotic Sensing and Human-robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300132, China. Electronic address: sli_mems@hebut.edu.cn.
J Colloid Interface Sci ; 678(Pt A): 1132-1142, 2025 Jan 15.
Article en En | MEDLINE | ID: mdl-39255752
ABSTRACT

HYPOTHESIS:

The co-flow step emulsification (CFSE) is very sensitive to the two-phase fluid interfaces, we conjecture that the CFSE hydrodynamic model depends on several key factors and the droplet generation process can be precisely controlled, thus to obtain droplet emulsions with the "ultra-high volume fraction of inner-phase" and "flexible droplet size" characteristics. The resulting droplets are expected to be applied to droplet digital PCR (ddPCR) with "high information density" and "wide dynamic range" advances. EXPERIMENTS By combining numerical simulation and fluid dynamics experiments, we have investigated the crucial parameters affecting the CFSE two-phase interface and finally achieved the prediction and guidance for CFSE droplet production.

FINDINGS:

With the help of the CFSE device, multivolume droplet populations were produced on demand. Then, ddPCR tests were performed with DNA concentrations from 10 copies/µL to 20,000 copies/µL. The CFSE device owns an ultra-wide dynamic range (up to 5 orders of magnitude), showing excellent quantification ability of nucleic acid targets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tamaño de la Partícula / Reacción en Cadena de la Polimerasa / Emulsiones / Dispositivos Laboratorio en un Chip Idioma: En Revista: J Colloid Interface Sci Año: 2025 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tamaño de la Partícula / Reacción en Cadena de la Polimerasa / Emulsiones / Dispositivos Laboratorio en un Chip Idioma: En Revista: J Colloid Interface Sci Año: 2025 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos