Factors influencing the uptake of DNA by Neurospora crassa.
Acta Biochim Biophys Acad Sci Hung
; 13(4): 259-67, 1978.
Article
en En
| MEDLINE
| ID: mdl-39418
ABSTRACT
Under optimal conditions intact Neurospora crassa cells incorporated nearly the same amount of 3H-labelled DNA as that of the endogenous DNA content of cells. After 18 h of incorporation more than 80 per cent of the radioactivity was retained in the cells. A maximum uptake of exogenous DNA occurred at 28 degrees C, pH 6.35, in the presence of 100 mM calcium when the concentration of donor DNA was 150 micrograms/ml. Denatured DNA was incorporated at a higher rate than native DNA. The present findings suggest that the uptake of DNA by Neurospora crassa is a biologically active process.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Neurospora
/
Neurospora crassa
Idioma:
En
Revista:
Acta Biochim Biophys Acad Sci Hung
Año:
1978
Tipo del documento:
Article