Separation of N1- and N8-acetylspermidine isomers by reversed-phase column liquid chromatography after derivatization with dansyl chloride.
J Chromatogr
; 375(1): 49-55, 1986 Feb 14.
Article
en En
| MEDLINE
| ID: mdl-3958109
ABSTRACT
The separation of dansyl derivatives of N1- and N8-acetylspermidine by reversed-phase column liquid chromatography is reported. The influence of organic solvents on the retention of acetylspermidines was studied. Best resolutions were achieved using a C18 column and a ternary mobile phase composed of water, methanol and acetonitrile. The precolumn derivatization method permitted the detection of picomole quantities. A method for the determination of acetylspermidines in rat tissues is described.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermidina
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr
Año:
1986
Tipo del documento:
Article