Further observations on the activation of lysosomal acid alpha-glucosidase by cortisone derivatives.
Biochem J
; 132(3): 435-8, 1973 Mar.
Article
en En
| MEDLINE
| ID: mdl-4724583
ABSTRACT
1. Cortisone acetate activates the acid alpha-glucosidase in rat liver slices and in isolated liver lysosomes. 2. The reaction is steroid specific and moreover does not occur with lysosomal acid phosphatase or beta-galactosidase. 3. After pretreatment of the lysosomes with cortisone, substrate (maltose) binding to the soluble lysosomal acid alpha-glucosidase is not affected, but the steroid does increase the V(max.) value. Membrane-bound enzyme is not activated by cortisone. 4. 4-[(14)C]Cortisone is preferentially bound to the lysosomal membrane and the possible involvement of this structure in the activation phenomenon is discussed.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cortisona
/
Glucosidasas
/
Lisosomas
Límite:
Animals
Idioma:
En
Revista:
Biochem J
Año:
1973
Tipo del documento:
Article