Size changes in single muscle fibers during fixation and embedding.
Tissue Cell
; 7(2): 383-7, 1975.
Article
en En
| MEDLINE
| ID: mdl-49940
ABSTRACT
During fixation of single muscles fibers with glutaraldehyde, the volume of the fiber shrinks 20%, recovers in rinse and osmium tetroxide to near normal volume and shrinks 20% again when staining with uranyl acetate. This suggest that osmotic properties of membranes may not have been completely lost during fixation, post-fixation and en bloc staining. Dehydration in ethanol and propylene oxide produces a further 10% shrinkage in volume. Infiltration and embedding with Epon causes an additional 15% change in volume. This gives a total shrinkage in volume of 45% which is nearly twice that of the apparent shrinkage in the volume of the myosin lattice as determined by electron microscopy.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Histológicas
/
Músculos
Límite:
Animals
Idioma:
En
Revista:
Tissue Cell
Año:
1975
Tipo del documento:
Article