Motility and eosin uptake of formaldehyde-treated ram spermatozoa.
J Reprod Fertil
; 65(2): 389-94, 1982 Jul.
Article
en En
| MEDLINE
| ID: mdl-6178824
ABSTRACT
A concentration of 0.005% formaldehyde in phosphate-buffered saline (PBS) achieved complete immobilization of ram spermatozoa while also yielding good recovery of sperm motility after removal by washing. At a higher formaldehyde concentration (0.01%) recovery rate declined with increasing dilution rate. Incubation of spermatozoa in PBS containing 0.005% formaldehyde beyond 6 h at 5, 15 or 25 degrees C resulted in poor recovery rates. Of the incubation temperatures, eosin uptake was lowest at 25 degrees C. During 4 h post-wash incubation at 30 degrees C sperm motility was significantly (P less than 0.001) affected by pre-wash formaldehyde concentration which had no effect on the proportion of eosinophilic spermatozoa.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
/
Espermatozoides
/
Eosina Amarillenta-(YS)
/
Formaldehído
Límite:
Animals
Idioma:
En
Revista:
J Reprod Fertil
Año:
1982
Tipo del documento:
Article