Improved genetically modified Escherichia coli strain for prescreening antineoplastic agents.
Antimicrob Agents Chemother
; 25(5): 622-5, 1984 May.
Article
en En
| MEDLINE
| ID: mdl-6203484
Clinical experience suggests that drugs that interact with and damage DNA are useful in cancer chemotherapy (H. Umezawa , p. 43-72, in V. T. DeVita , Jr., and H. Busch [ed.], Methods in Cancer Research; Cancer Drug Development, vol. XVI, 1979). Prescreening systems for antitumor agents in natural products require assays that are exquisitely sensitive, since the active components are often produced in quantities of micrograms per milliliter or less. One assay used to identify agents that interact with DNA is the biochemical induction assay, utilizing Escherichia coli BR 513 (R. K. Elespuru and R. J. White, Cancer Res. 43:2819-2830, 1983). In this paper we describe a genetic modification of strain BR 513 that displays an expanded spectrum of activity. This strain may provide an improved prescreen for detecting natural products that interact with DNA.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Escherichia coli
/
Antibióticos Antineoplásicos
Idioma:
En
Revista:
Antimicrob Agents Chemother
Año:
1984
Tipo del documento:
Article
Pais de publicación:
Estados Unidos