Immunofluorescent staining of Treponema in tissues fixed with formalin.
Arch Pathol Lab Med
; 108(11): 878-80, 1984 Nov.
Article
en En
| MEDLINE
| ID: mdl-6207797
ABSTRACT
Immunofluorescent examination of formalin-fixed tissue for Treponema pallidum has generally been unsatisfactory because of nonspecific background fluorescence and poor contrast. We examined the process of treating deparaffinized formalin-fixed tissue sections with 1% ammonium hydroxide (NH4OH) to improve fluorescent staining. Treponema pallidum- and Treponema pertenue-infected rabbit testes or human tissue biopsy specimens fixed in 10% buffered formalin and embedded in paraffin were examined. Sections were cut one week to five years after embedment. Tissues were then stained with fluorescein- or rhodamine-labeled human anti- T pallidum globulin for 30 minutes at 37 degrees C. Treponemes were consistently stained and background staining was generally reduced after NH4OH treatment in both fresh and stored tissue. Cutting sections at a thickness of approximately 2 micron was critical to achieve optimal fluorescence.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones por Treponema
/
Técnicas Histológicas
/
Formaldehído
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Arch Pathol Lab Med
Año:
1984
Tipo del documento:
Article