Photoaffinity labeling of insulin receptor of rat adiopocyte plasma membrane.
J Biol Chem
; 253(6): 1743-5, 1978 Mar 25.
Article
en En
| MEDLINE
| ID: mdl-632238
ABSTRACT
A photosensitive insulin derivative was synthesized by reacting radioactive iodinated bovine insulin with N-hydroxysuccinimide ester of 4-azidobenzolic acid. The photo-sensitivity and specificity of this insulin derivative were established by its covalent nonspecific cross-link to albumin and its covalent specific cross-link to the heavy and light chains of anti-insulin immunoglobulin. Plasma membrane preparations of rate adipocytes were incubated with the photosensitive insulin derivative and irradiated with light. Sodium dodecyl sulfate gel electrophoresis of these plasma membrane preparations after solubilization with sodium dodecyl sulfate and reduction with beta-mercaptoethanol showed that a protein having a molecular weight of 130,000 was specifically labeled by the radioactive photosensitive insulin, suggesting that this protein may be the insulin receptor.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptor de Insulina
/
Tejido Adiposo
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
1978
Tipo del documento:
Article