High-speed bubble-segmented blood sampler for the rat.
J Appl Physiol Respir Environ Exerc Physiol
; 57(4): 1284-7, 1984 Oct.
Article
en En
| MEDLINE
| ID: mdl-6501037
An arterial blood sampler for use in the conscious rat is described. With this apparatus it is possible to obtain small (10 microliter) whole-blood or plasma samples as frequently as 1/s and to derive accurate arterial time-concentration curves in the first 60-120 s after compounds are injected for regional blood flow or pharmacokinetic measurements. The blood is withdrawn from an implanted arterial catheter into polyethylene tubing at a constant rate while bubbles are introduced at regular intervals via a side channel into the column of blood. Although some dispersion of blood samples occurs as the tubing is traversed, this can be mathematically corrected. However, correction is unnecessary if the information of interest is the area under the time-concentration curve.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recolección de Muestras de Sangre
Límite:
Animals
Idioma:
En
Revista:
J Appl Physiol Respir Environ Exerc Physiol
Año:
1984
Tipo del documento:
Article
Pais de publicación:
Estados Unidos