Specific binding of [3H]oxytocin in the female rat brain.
Can J Physiol Pharmacol
; 61(9): 989-95, 1983 Sep.
Article
en En
| MEDLINE
| ID: mdl-6640435
ABSTRACT
Because of demonstrated effects of oxytocin on some limbic system mediated behaviours, the specific binding of [3H]oxytocin to a plasma membrane containing fraction of rat limbic tissue has been studied. The binding of the microsomal fraction of estrogenized, female rat tissue was time dependent and saturable, with a Bmax of 2.5 X 10(-13) moles per milligram of protein and an apparent KD of 3.53 X 10(-8)M, and appeared to show positive cooperativity. The pH optimum of the binding was 6.0, close to the pH optimum for oxytocin-neurophysin binding; however, other results show the two types of binding to be different. The microsomal fraction did not appreciably degrade oxytocin under the conditions used for [3H]oxytocin binding. The distribution in limbic tissue of oxytocin-degrading activity and of individual enzymes capable of degrading oxytocin has been examined and an interplay of enzymes concentrated in different cell types is proposed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Encéfalo
/
Oxitocina
/
Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
Can J Physiol Pharmacol
Año:
1983
Tipo del documento:
Article