Microtubules and microfilaments in ageing hamster embryo fibroblasts in vitro.
Exp Gerontol
; 18(3): 241-54, 1983.
Article
en En
| MEDLINE
| ID: mdl-6685652
ABSTRACT
Microtubules and microfilaments were investigated in hamster lung fibroblasts, during their in vitro life-span. These cells show a senescence process characterized by a drastic phenotypic change, resulting in two phenotypes the type 1 cells, characteristic of young cultures and the type 2 cells appearing progressively with culture passages. Microtubules and microfilaments were observed at the TEM and also visualized by the unlabelled peroxidase-anti-peroxidase method. Moreover, the susceptibility of microtubules to nocodazole was tested in type 1 and 2 cells. We could not provide evidence for a different susceptibility to the drug. However the depolymerization wave occurred centripetally in type 1 cells whilst centrifugally in type 2 cells. These observations are discussed in relationship with the early arrest of division growth of the type 2 differentiated cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citoesqueleto
/
Supervivencia Celular
/
Células Cultivadas
/
Microtúbulos
Límite:
Animals
Idioma:
En
Revista:
Exp Gerontol
Año:
1983
Tipo del documento:
Article