Synthesis and properties of N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine, a new reagent for RNA-RNA and RNA-protein cross-linking.
Eur J Biochem
; 103(2): 365-75, 1980 Jan.
Article
en En
| MEDLINE
| ID: mdl-6988212
ABSTRACT
The synthesis of N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine (Gbz-Cyn2-Ac) is described. Like other glyoxal-type reagents it reacts with guanine and arginine. The kinetics and pH dependence of these reactions are studied. (Gbz-Cyn2-Ac) reacts with non-base-paired guanines at about 20 sites in 16-S rRNA. After reduction of disulfide bonds each derivatized guanine residue carries a free -- SH group which can be used to create RNA-RNA bridges or, after introducing an additional photoactivatable derivative, RNA-protein bridges.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Ribosómicas
/
ARN Ribosómico
/
Reactivos de Enlaces Cruzados
/
Cistamina
Idioma:
En
Revista:
Eur J Biochem
Año:
1980
Tipo del documento:
Article