Determination of avermectins in plasma at nanogram levels using high-performance liquid chromatography with fluorescence detection.
J Chromatogr
; 190(2): 367-76, 1980 Apr 04.
Article
en En
| MEDLINE
| ID: mdl-7380953
ABSTRACT
An analytical method is described for the determination of the avermectins in plasma based upon high-performance liquid chromatography of fluorescent derivatives of these compounds. The analyte is isolated by adsorption chromatography on Florisil, dehydrated in an acetic anhydride-pyridine mixture, and the fluorophore is further separated by chromatography on silica gel in advance of introduction into a reversed-phase system. This method, which can be applied to samples containing as little as 0.2 ng drug per ml, has an accuracy of 5% mean relative error and a precision of 8% relative standard deviation. A study and discussion of several factors which affect the analytical reaction are included.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ivermectina
/
Lactonas
/
Antihelmínticos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr
Año:
1980
Tipo del documento:
Article