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The transposable element mariner can excise in non-drosophilid insects.
Coates, C J; Turney, C L; Frommer, M; O'Brochta, D A; Warren, W D; Atkinson, P W.
Afiliación
  • Coates CJ; Commonwealth Scientific and Industrial Research Organisation, Division of Entomology, Canberra, Australia.
Mol Gen Genet ; 249(2): 246-52, 1995 Nov 15.
Article en En | MEDLINE | ID: mdl-7500947
ABSTRACT
Plasmid-based excision assays performed in embryos of two non-drosophilid species using the mariner transposable element from Drosophila mauritiana resulted in empty excision sites identical to those observed after the excision of mariner from D. mauritiana chromosomes. In the presence of the autonomous mariner element Mos1, excision products were recovered from D. melanogaster, D. mauritiana and the blowfly Lucilia cuprina. When a hsp82 heat shock promoter-Mos1 construct was used to supply mariner transposase, excision products were also recovered from the Queensland fruitfly Bactrocera tryoni. Analysis of DNA sequences at empty excision sites led us to hypothesise that the mariner excision/repair process involves the formation of a heteroduplex at the excision breakpoint. The success of these assays suggests that they will provide a valuable tool for assessing the ability of mariner and mariner-like elements to function in non-drosophilid insects and for investigating the basic mechanisms of mariner excision and repair.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elementos Transponibles de ADN / Dípteros / Drosophila / Drosophila melanogaster Límite: Animals Idioma: En Revista: Mol Gen Genet Año: 1995 Tipo del documento: Article País de afiliación: Australia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elementos Transponibles de ADN / Dípteros / Drosophila / Drosophila melanogaster Límite: Animals Idioma: En Revista: Mol Gen Genet Año: 1995 Tipo del documento: Article País de afiliación: Australia