Glutamate- and AMPA-mediated calcium influx through glutamate receptor channels in medial septal neurons.
Neuropharmacology
; 32(11): 1221-8, 1993 Nov.
Article
en En
| MEDLINE
| ID: mdl-7509048
ABSTRACT
The Ca(2+)-fraction of the ion current flowing through glutamate receptor channels activated either by glutamate or by AMPA was determined in forebrain neurons of the rat medial septum. By combining whole-cell patch-clamp and fura-2 fluorometric measurements we found that, at negative membrane potentials and at an extracellular free Ca(2+)-concentration of 1.6 mM, the Ca(2+)-fraction of the current activated by glutamate is 5.7%. A pharmacological analysis of responses produced by ionophoretically-released glutamate demonstrated a large contribution of NMDA-receptors but a small contribution of AMPA/kainate receptors to these responses. Interestingly, also AMPA-mediated currents were associated with significant changes in Ca(2+)-sensitive fluorescence. The fractional Ca2+ current of AMPA-induced responses was 1.2 +/- 0.4% (n = 5).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Canales de Calcio
/
Calcio
/
Receptores de Glutamato
/
Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico
/
Glutamatos
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Neuropharmacology
Año:
1993
Tipo del documento:
Article
País de afiliación:
Alemania