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Phosphotryptic peptide analysis of the human androgen receptor: detection of a hormone-induced phosphopeptide.
Kuiper, G G; Brinkmann, A O.
Afiliación
  • Kuiper GG; Department of Endocrinology and Reproduction, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands.
Biochemistry ; 34(6): 1851-7, 1995 Feb 14.
Article en En | MEDLINE | ID: mdl-7531494
Phosphorylation of the androgen receptor (AR) in human prostate tumor cells (LNCaP) is increased by androgens. The AR is expressed as two isoforms with apparent molecular masses of 110 and 112 kDa. Metabolic labeling experiments with [32P]orthophosphate revealed that only the 112 kDa isoform is radioactively labeled. Phosphoamino acid analysis revealed only phosphorylation on serine residues. Phosphotryptic peptide analysis of human AR protein by two-dimensional peptide mapping and by reverse-phase HPLC showed phosphorylation at multiple sites. Comparison of phosphopeptide maps of AR protein from cells incubated in the absence or presence of the synthetic androgen R1881 indicated that the ligand-stimulated phosphorylation is probably due to induction of phosphorylation at a new site rather than increased phosphorylation at an existing site. This result suggests that hormone-dependent AR phosphorylation might play a role in the signal transduction pathway of androgens.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoproteínas / Tripsina / Receptores Androgénicos / Andrógenos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Biochemistry Año: 1995 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoproteínas / Tripsina / Receptores Androgénicos / Andrógenos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Biochemistry Año: 1995 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos