A mutation in the epidermal growth factor receptor in waved-2 mice has a profound effect on receptor biochemistry that results in impaired lactation.
Proc Natl Acad Sci U S A
; 92(5): 1465-9, 1995 Feb 28.
Article
en En
| MEDLINE
| ID: mdl-7533293
ABSTRACT
The mutant mouse waved-2 (wa-2) is strikingly similar to transforming growth factor alpha-deficient mice generated by gene targeting in embryonic stem cells. We confirm that wa-2 is a point mutation (T-->G resulting in a valine-->glycine substitution at residue 743) in the gene encoding the epidermal growth factor (EGF) receptor. wa-2 fibroblastic cells lack high-affinity binding sites for EGF, and the rate of internalization of EGF is retarded. Although the tyrosine kinase activity of wa-2 EGF receptors is significantly impaired, NIH 3T3 cells lacking endogenous EGF receptors but overexpressing recombinant wa-2 EGF receptor cDNA are mitogenically responsive to EGF. While young and adult wa-2 mice are healthy and fertile, 35% of wa-2 mice born of homozygous wa-2 mothers die of malnutrition because of impaired maternal lactation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lactancia
/
Proteínas Tirosina Quinasas Receptoras
/
Factor de Crecimiento Epidérmico
/
Receptores ErbB
Límite:
Animals
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
1995
Tipo del documento:
Article
País de afiliación:
Australia