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Endothelium-modulated proliferation of medial smooth muscle cells: influence of angiotensin II and converting enzyme inhibition.
Hahn, A W; Schmidt, R; Kern, F; Resink, T J; Bühler, F R.
Afiliación
  • Hahn AW; Department of Research, University Clinics Basel, Switzerland.
Eur Heart J ; 16 Suppl C: 29-32, 1995 May.
Article en En | MEDLINE | ID: mdl-7556269
This study investigated the role of the endothelium and angiotensin II (Ang II) in regulating medial smooth muscle cell (SMC) proliferation. [3H]-thymidine incorporation into medial SMC of rat arteries was examined in vivo, using ballooned rat carotid arteries, as well as in vitro, using cultures of aortic tissue rings (organoids). In vivo, maximal medial [3H]-thymidine incorporation occurred within 3 days post-ballooning. In endothelium-denuded organoids, maximum medial DNA synthesis was achieved after 7 days of culture. [3H]-thymidine-labelling of SMC in intact organoids (with endothelium) increased minimally during culture, indicating that the endothelium provided protection with respect to medial proliferation under basal conditions (culture in the presence of 1% plasma-derived serum). Inclusion of 10(-7) M Ang II significantly elevated medial [3H]-thymidine incorporation above that in control cultures. The stimulatory effect of Ang II was much more pronounced in intact organoids that in endothelium-denuded organoids, indicating synergistic growth regulation by Ang II and endothelium-derived factors. When organoids were cultured in the combined presence of Ang II and the ACE inhibitor cilazaprilat, labelling indices of intact organoids were also significantly increased above control, but to a lower level than those obtained in the presence of Ang II alone. However, for endothelium-denuded organoids, medial [3H]-thymidine incorporation in the combined presence of Ang II and cilazaprilat was not significantly different from that in untreated controls. Thus, cilazaprilat exerts both endothelium-dependent and endothelium-independent negative regulatory effects on medial SMC proliferation.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Angiotensina II / Inhibidores de la Enzima Convertidora de Angiotensina / Endotelio Vascular / Músculo Liso Vascular Límite: Animals Idioma: En Revista: Eur Heart J Año: 1995 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Angiotensina II / Inhibidores de la Enzima Convertidora de Angiotensina / Endotelio Vascular / Músculo Liso Vascular Límite: Animals Idioma: En Revista: Eur Heart J Año: 1995 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido