Biotinylated L-selectin ligand analogs as cytochemical probes in cytospin preparations.
Folia Histochem Cytobiol
; 33(1): 39-41, 1995.
Article
en En
| MEDLINE
| ID: mdl-7556772
ABSTRACT
Amino-derivatives of L-selectin ligand analogs phosphonoester core polysaccharide (PPME) and fucoidin were biotinylated with the use of biotinyl-N-succinimide ester, and these biotinylated analogs b-PPME and b-fucoidin were demonstrated as useful tools to investigate the functional activity of L-selectins in cytospin preparations obtained from healthy human donors and from patients with chronic lymphocytic leukemia (CLL). The avidin/biotin system adds a new alternative to the application of the L-selectin ligand analogs (PPME, fucoidin) which have been formerly used as fluoresceinated, solid phase or immobilized probes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Marcadores de Spin
/
Linfocitos
/
Selectina L
/
Histocitoquímica
/
Mananos
/
Manosafosfatos
Límite:
Humans
Idioma:
En
Revista:
Folia Histochem Cytobiol
Asunto de la revista:
HISTOCITOQUIMICA
Año:
1995
Tipo del documento:
Article
País de afiliación:
Hungria