Mineral fiber-induced leukocyte activation: the role of intra- and extracellular calcium.
Toxicol Lett
; 78(3): 195-205, 1995 Aug.
Article
en En
| MEDLINE
| ID: mdl-7624890
ABSTRACT
The role of intra- and extracellular calcium in the activation of human polymorphonuclear leukocytes (PMNL) to produce reactive oxygen metabolites (ROM) were studied by using soluble, formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), or particulate stimuli, quartz or chrysotile. A calcium channel inhibitor, verapamil, attenuated only quartz-induced elevation of free intracellular calcium ([Ca2+]i) and ROM production. Likewise, ethyleneglycol-bis (aminoethyl ether) tetraacetic acid (EGTA) attenuated quartz-, chrysotile- and fMLP-induced elevation of [Ca2+]i and ROM production. It also inhibited PMA-induced ROM production. A calcium ionophore, A23187 amplified ROM production by all of these stimuli. These results suggest that both intra- and extra-cellular calcium are required for the full activation of respiratory burst by soluble and particulate stimuli in human PMNL.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cuarzo
/
Activación de Linfocitos
/
Calcio
/
Asbestos Serpentinas
/
Neutrófilos
Límite:
Humans
Idioma:
En
Revista:
Toxicol Lett
Año:
1995
Tipo del documento:
Article
País de afiliación:
Finlandia